Thermo Fisher Scientific CENTB1 Polyclonal Antibody
Rabbit polyclonal antibody targeting CENTB1/ACAP1 protein. Validated for Western blot at 1.0–2 µg/mL. Synthetic peptide immunogen. Supplied as liquid, 0.5 mg/mL concentration. Suitable for research use only.
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Thermo Fisher Scientific CENTB1 Polyclonal Antibody
Applications
- Western Blot (WB)
Tested Dilution: 1.0–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide (535 RPRGQPPVPPKPSIR 549) of CENTB1/ACAP1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2540867 |
Target Information
ACAP1, also known as Centaurin-beta-1 (CENTB1), is a 740 amino acid transport carrier belonging to the Centaurin family. It contains three ANK repeats, an Arf-GAP domain, a BAR domain, and a PH domain that binds phospholipids.
ACAP1 acts as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) and is required for clathrin-dependent export of proteins from recycling endosomes to the trans-Golgi network and cell surface.
It mediates CENTB1 binding to phosphatidylinositol 3,5-bisphosphate (PIP2) or phosphatidylinositol 3,4,5-trisphosphate (PIP3) containing membranes.
Together with ACAP2, ACAP1 is recruited to PDGF-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, while overexpression inhibits ruffle formation.
ACAP1 is widely expressed in most tissues, with high expression in lung and spleen and lower levels in heart, kidney, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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