Thermo Fisher Scientific BAX Polyclonal Antibody
Thermo Fisher Scientific의 BAX Polyclonal Antibody는 인간 BAX 단백질을 인식하는 rabbit IgG 기반 항체로, apoptosis 관련 연구에 적합합니다. Western blot 및 IHC(P, PFA)에서 검증되었으며, 항원 친화 크로마토그래피로 정제된 고품질 시약입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2–10 µg/mL
Immunohistochemistry (PFA fixed) (IHC (PFA))
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to N-term of human BAX protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: A375, HeLa, or Hodgkin’s lymphoma
- Cellular location: Cytoplasmic and cell membrane
Target Information
BAX is a member of the Bcl-2 family and plays a crucial role in the regulation of apoptosis. While Bcl-2 acts as an anti-apoptotic protein, BAX functions as a pro-apoptotic factor. Apoptosis regulation involves homo- and heterodimerization of BAX and Bcl-2 isoforms.
The Bax gene encodes multiple isoforms, including Bax alpha (21 kDa) and Bax beta (24 kDa). Both contain BH1, BH2, and BH3 domains, but Bax beta lacks a hydrophobic transmembrane domain. Bcl-2 also exists in alpha and beta isoforms, differing in sequence and size.
Overexpression of BAX promotes cell death by forming homodimers or heterodimers with other BCL-2 related proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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