Thermo Fisher Scientific RNF169 Polyclonal Antibody
RNF169 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ELISA에 사용 가능. Human, Mouse, Rat에 반응하며, 친화 크로마토그래피로 정제됨. 동결건조 형태로 제공되며, 재구성 후 500 µg/mL 농도 유지. DNA 손상 복구 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human RNF169 recombinant protein (Position: N311–K708) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2884854 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage.
Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSB repair.
E3 ubiquitin-protein ligase activity is not required for regulation of DSB repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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