Thermo Fisher Scientific ATP5G1/2/3 Polyclonal Antibody
ATP5G1/2/3 단백질을 인식하는 Thermo Fisher의 Rabbit Polyclonal 항체. WB, IHC, ICC/IF, Flow, ELISA에 적합. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
| ELISA | 0.1–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human ATP5G1,2,3/ATP5MC1,2,3 recombinant protein (Position: D62–L113) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2884853 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase, mapped to 17q21.32.
This gene encodes a subunit of mitochondrial ATP synthase, which catalyzes ATP synthesis utilizing an electrochemical proton gradient across the inner membrane during oxidative phosphorylation.
ATP synthase consists of two linked multi-subunit complexes: the soluble catalytic core (F1) and the membrane-spanning proton channel (Fo).
The catalytic portion includes five subunits (α, β, γ, δ, ε) with a 3α:3β:1 stoichiometry, while the proton channel contains nine subunits (a, b, c, d, e, f, g, F6, and 8).
Each of the three genes encoding subunit c has distinct mitochondrial import sequences but produces the same mature protein.
Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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