Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Yellow 590, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.1 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780, Biotin, eFluor 450, FITC
• Functional Grade, NovaFluor Blue/Red/Yellow/Violet series
• PE, PE-Cyanine5, PE-Cyanine7, PerCP-eFluor 710
• Super Bright series
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with human CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers. CD8a is found on most thymocytes and subsets of mature T and NK cells. CD8 binds to MHC class I and, via p56lck kinase association, contributes to T-cell development and activation.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.
This antibody is optimized for flow cytometric analysis and pre-titrated for use at 5 µL (0.1 µg)/test in a 100 µL final volume. Cell count per test may range from 10⁵ to 10⁸.

Note:

• NovaFluor dyes are not compatible with DNA intercalating dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Product # C001T06F01) is required for optimal staining. Add 5 µL per sample before combining with cells.

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures designed to minimize spillover and spread.
Excitation: 552 nm; Emission: 592 nm; Laser: 561 nm (Yellow)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction, T-cell differentiation, and activation.

• Expressed primarily on T cells as CD8αβ heterodimers, functioning as a co-receptor for MHC class I molecules.
• CD8α chain binds directly to MHC-I, while CD8αα homodimers are found on subsets of T cells, NK cells, monocytes, and dendritic cells.
• Ligation with MHC-I/peptide complexes triggers Lck kinase recruitment, lymphokine production, and cytotoxic T lymphocyte (CTL) activation.
• Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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