Thermo Fisher Scientific MAP2K3 Monoclonal Antibody (OTI4B3), TrueMAB
MAP2K3 단백질을 표적하는 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 개, 마우스 반응성. HEK293T 세포에서 생산된 MAP2K3 전체 길이 단백질을 면역원으로 사용. 동결건조 형태로 제공되며, PBS 버퍼에 trehalose 포함. 연구용 전용.
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Thermo Fisher Scientific MAP2K3 Monoclonal Antibody (OTI4B3), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B3 |
| Immunogen | Full length human recombinant protein of human MAP2K3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction. It phosphorylates and activates MAPK14/p38-MAPK and plays a role in glucose transporter expression. Activation can occur via insulin or RAS oncogene expression, leading to constitutive MAPK14 activation and oncogenic transformation. Inhibition of MAP2K3 is linked to the pathogenesis of Yersinia pseudotuberculosis. Multiple alternatively spliced transcript variants exist for MEK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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