Thermo Fisher Scientific Resistin (RETN) Monoclonal Antibody (RETN, 3331)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature (RT).
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at RT for 20 minutes.

Target Information

Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin.
Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.
Studies show that in obese, diabetic mice, serum resistin levels are increased, which antagonizes insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice.
Resistin gene expression requires C/EBP alpha for activation. Coactivators CBP and p300, which display histone acetyltransferase (HAT) activity, acetylate histones at the resistin promoter, suggesting their role in activating chromatin for resistin transcription.
Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.