Thermo Fisher Scientific DCP1A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Signal transduction of TGF‑beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF‑beta‑induced transcriptional activation wherein phosphorylated receptor‑associated Smads associate with Smad4. Furthermore, SMIF (Smad4‑interacting protein) and Smad4 complex with TGF‑beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through an EVH1/WH1 domain on SMIF and a proline‑rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF, as deletion of the Smad4‑interacting domain (located in the N‑terminal 100 amino acids) of SMIF eliminates TGF‑beta‑induced nuclear translocation of SMIF. The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.