Thermo Fisher Scientific HACE1 Monoclonal Antibody (OTI4E10), TrueMAB
HACE1 단백질을 인식하는 마우스 단클론 항체로, Western blot 등에 사용 가능. 사람, 생쥐, 랫트 반응성. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도 권장. Golgi 막 융합 및 GTPase 조절 관련 연구에 적합.
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Applications
- Western Blot (WB): 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E10 |
| Immunogen | Full length human recombinant protein of human HACE1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases.
- Regulates Golgi membrane dynamics during the cell cycle by interacting with Rab proteins.
- Mediates ubiquitination during mitotic Golgi disassembly, serving as a signal for reassembly post-mitosis.
- Interacts specifically with GTP-bound RAC1, mediating its ubiquitination and degradation, contributing to host defense.
- May also act as a transcription regulator through interaction with RARB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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