Thermo Fisher Scientific IL3RA/CD123 Monoclonal Antibody (IL3RA, 1531)
인간 IL3RA/CD123 단백질을 인식하는 mouse monoclonal antibody. IHC(P) 및 peptide array에 사용 가능. Protein A/G로 정제된 액상형 항체로 4°C 보관. AML 세포에서 과발현되는 IL-3 수용체 α사슬 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IL3RA, 1531 |
| Immunogen | Recombinant fragment of human IL3RA protein (around aa 26–171) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and forms a heterodimer with the common beta (βc or CDw131) subunit.
IL-3Ra mediates the signal transduction of Interleukin-3. Heterodimerization of IL-3Ra and IL-3Rb chains is induced by Interleukin-3, which is necessary for receptor activation but not for high-affinity binding.
IL-3Ra is reported to be overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells, making it a potential target for novel therapeutic agents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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