Thermo Fisher Scientific SaCas9 Monoclonal Antibody (11C12), GenCRISPR

Thermo Fisher Scientific SaCas9 Monoclonal Antibody (11C12), GenCRISPR

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
• The CRISPR/Cas9 system provides a robust and multiplexable genome editing tool, enabling precise manipulation of specific genomic elements and facilitating gene function studies in biology and disease.
• The Cas9 proteins from Streptococcus pyogenes and Staphylococcus aureus are well-characterized CRISPR-associated nucleases.
• Accurate control of transfection efficiency and Cas9 expression levels is critical during genome editing, achievable with specific anti-CRISPR/Cas9 antibodies.

Target Information

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR adaptive immunity system in bacteria and genetic engineering.
This system uses RNA to guide the nuclease to a desired DNA sequence, allowing for targeted indel mutations, sequence insertions, deletions, or rearrangements.

SaCas9, derived from Staphylococcus aureus, offers several advantages for genome editing:

• Small size and high efficiency
• Nickase activity and high specificity
• Recognizes NNGRRT PAM sequence
• Comparable cleavage rate to SpCas9 with spacer lengths of 20–24 nucleotides

Compared with SpCas9, SaCas9 (1053 AA) is smaller than SpCas9 (1368 AA) and provides efficient indel generation.
It is preferred for all-in-one delivery systems using adeno-associated vectors for Cas9 and multiple gRNA expression cassettes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.