Thermo Fisher Scientific Rabbit anti-Human IgG Fc Secondary Antibody, FITC

Applications

Tested Dilution

Immunohistochemistry (IHC)

1:50 - 1:200

Immunocytochemistry (ICC/IF)

1:50 - 1:200

Flow Cytometry (Flow)

1:50 - 1:200

Immunoprecipitation (IP)

1:50 - 1:200

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

FITC FITC FITC

View additional formats

• [AP](/antibody/secondary/product-name/Human IgG Fc/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Biotin/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Human IgG Fc/conjugates/HRP/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_228408

Target

IgG Fc

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Antibody Specificity: This antibody reacts with the heavy chains of human IgG, but not with the light chains on most human immunoglobulins. No antibody was detected against human IgM, IgA, or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1)

Amax = 492 nm; Emax = 520 nm

Fluorophore/Protein: 7.5 µg/mg; 2.8 moles FITC per mole IgG

Restoration and Storage: Store product at 4°C until opened. Restore with 1.1 mL distilled water (1.5 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.

To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Country of Origin: USA

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.