Thermo Fisher Scientific beta Actin Polyclonal Antibody
Thermo Fisher Scientific의 beta Actin Polyclonal Antibody는 다양한 종에서 반응하며 Western blot, IHC, ICC 등 다중 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 고품질 항체로, 세포골격 연구 및 단백질 로딩 컨트롤에 이상적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:20,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Fruit fly, Horse, Cat, Hamster, Human, Mouse, Pig, Rat, Xenopus, Yeast |
| Published Species | Dog |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.26 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792414 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).
- Positive Control: 293T, A341, Jurkat, Raji, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH-3T3, mouse brain, PC-12, Rat2, Rat brain, drosophila, Yeast lysate, Candida albicans (sc5314) whole cell lysate, Rice leaf, mouse aorta, H9C2, OKT3, mouse ileum tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue, human periodontal ligament.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein expressed in all human cell types and involved in cell motility, structure, and integrity. Beta and gamma actin coexist in most cell types as components of the cytoskeleton. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) with a two-stranded helical structure.
Because beta actin is ubiquitously expressed, it is commonly used as a loading control in protein detection assays such as Western blotting. Antibodies to beta actin are useful for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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