Thermo Fisher Scientific HLA-Aw32 and HLA-A25 (MHC I) Monoclonal Antibody (SPM417)

Applications and Tested Dilution

Product Specifications

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.

The heavy chain is approximately 45 kDa and its gene contains 8 exons:

• Exon 1 encodes the leader peptide
• Exons 2 and 3 encode the alpha1 and alpha2 domains (peptide binding)
• Exon 4 encodes the alpha3 domain
• Exon 5 encodes the transmembrane region
• Exons 6 and 7 encode the cytoplasmic tail

Polymorphisms within exon 2 and exon 3 determine peptide binding specificity. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies play a significant role in immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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