Thermo Fisher Scientific HLA-Aw32 and HLA-A25 (MHC I) Monoclonal Antibody (SPM417)

Applications and Tested Dilution

Product Specifications

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane and plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen.

They are expressed in nearly all cells. The heavy chain (~45 kDa) gene contains 8 exons:

• Exon 1 encodes the leader peptide
• Exons 2 and 3 encode the alpha1 and alpha2 domains (peptide binding)
• Exon 4 encodes the alpha3 domain
• Exon 5 encodes the transmembrane region
• Exons 6 and 7 encode the cytoplasmic tail

Polymorphisms within exon 2 and exon 3 determine peptide-binding specificity. Typing for these polymorphisms is routinely performed in bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are essential tools in Immunopeptidomics, enabling identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.