Thermo Fisher Scientific HLA-Aw32 and HLA-A25 (MHC I) Monoclonal Antibody (SPM418)

Applications and Tested Dilution

Product Specifications

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane and plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. It is expressed in nearly all cells.

The heavy chain (~45 kDa) gene contains 8 exons:

• Exon 1: leader peptide
• Exons 2–3: α1 and α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2 and 3 determine peptide-binding specificity. Typing for these polymorphisms is routinely used in bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are critical in immunopeptidomics, enabling identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.