Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), Super Bright 436, eBioscience

Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 forms heterodimers with alpha integrins (CD49a–f) to create VLA complexes (VLA-1 through VLA-6) and interacts with CD51. These complexes mediate cellular adhesion, trafficking, proliferation, and differentiation.

CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin, and interact with receptors including VCAM-1 and MadCAM-1.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤0.5 µg/test). A test corresponds to staining a cell sample in 100 µL volume. Cell number typically ranges from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Fluorophore Information:
Super Bright 436 is excited at 405 nm and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

ITGB1 (Integrin Subunit Beta 1, CD29):
A 110 kDa cell surface glycoprotein expressed by various cells including all leukocytes. Forms heterodimers with alpha chains (α1–α6), determining the binding properties of VLA integrins. Plays a role in platelet adhesion, immune cell trafficking, and extracellular matrix interactions. Decreased ITGB1 expression is associated with multidrug resistance in tumor cells. Upregulated during leukocyte migration and inflammation, influencing cytokine secretion and tissue response.

Two major isoforms exist: β1A (ubiquitous except muscle) and β1D (cardiac and skeletal muscle).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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