Thermo Fisher Scientific NEFL Polyclonal Antibody
NEFL 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성 보유하며, 고순도 항원 친화 크로마토그래피로 정제되어 안정적 액상 형태로 제공됩니다.
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Thermo Fisher Scientific NEFL Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 298–525 of 68 kDa Neurofilament Light |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.09 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552002 |
Product Specific Information
- Recommended positive controls: human brain, PC-3, U87-MG, SK-N-SH, mouse cerebellum
- Predicted reactivity: Rhesus Monkey (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Neurofilaments are intermediate filament proteins specific to neurons, composed mainly of NF-L, NF-M, and NF-H.
NF-L is the light or low molecular weight subunit (~70 kDa), while NF-H is the heavy subunit (~180–220 kDa).
Their expression is tissue-specific and developmentally regulated, contributing to the maintenance of neuronal caliber.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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