Thermo Fisher Scientific PP2A alpha Polyclonal Antibody
PP2A alpha 단백질 인산화 조절 연구용 토끼 폴리클로날 항체. Western blot, IHC, ICC, IP 등 다양한 응용에 적합. 인간, 생쥐, 랫드 등 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 보존제 미함유.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:400 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | View 1 publication |
| Immunoprecipitation (IP) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Non-human primate, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids at the carboxy terminus of human PP2A catalytic subunit |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 27 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10983779 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other PP2A subunits.
Target Information
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis, and apoptosis.
A group of proteins that are intimately involved in this process are the protein phosphatases.
In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit, and a catalytic subunit.
Four major families of protein phosphatase catalytic subunits have been identified: PP1, PP2A, PP2B (calcineurin), and PP2C.
The PP2A family comprises subfamily members PP2A alpha and PP2A beta.
An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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