Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Brilliant Ultra Violet 496, eBioscience

Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Brilliant Ultra Violet 496, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test
Publications: - (none listed)

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 661 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1-positive strains (C57BL, NZB). Common strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1.
For NK cell detection in these strains, use the DX5 monoclonal antibody (CD49b, Product #14-5971).

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression in most cells, with minor DX5+PK136− and DX5−PK136+ populations.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometry of mouse splenocytes, ≤1.0 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm, excited by UV (355 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product #SB-4401-42)
• Brilliant Stain Buffer™ (Product #00-4409-75)

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples may be stored up to 3 days in the dark at 4°C using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

NK1.1 (KLRB1, NKR-P1A, CD161) is expressed by Natural Killer (NK) cells and some T lymphocytes. It belongs to the C-type lectin superfamily and plays a role in NK cell regulation.
The KLRB1 protein includes extracellular C-type lectin motifs, a transmembrane domain, and a cytoplasmic domain.
NKR-P1A recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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