Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet 496, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet 496, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Species Reactivity

Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700, Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0). Ki-67 serves as a marker for cell proliferation and is used to determine the growth fraction of cell populations, particularly in tumor studies.

This antibody also reacts with human, non-human primate, and canine Ki-67.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
• Recommended Usage: ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimization: Titrate antibody for best performance

Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is suitable for cytometers with a 355 nm UV laser.
Use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize polymer dye interactions.

• Light Sensitivity: Protect from light to prevent photo-induced oxidation
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) up to 3 days at 4°C in the dark
• Excitation: 350 nm
• Emission: 496 nm
• Laser: Ultraviolet

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during G1, S, G2, and M phases of the cell cycle, but absent in G0. It associates with euchromatin and heterochromatin and is widely used as a proliferation marker. Ki-67 index correlates with tumor grade and is valuable in cancer prognosis and neuronal proliferation studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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