Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Ultra Violet 496, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Ultra Violet 496, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488, 532, 561, 647
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395, 563, 615
• Brilliant Violet 421, 480, 605, 711
• eFluor 450, 506, FITC, Functional Grade
• Pacific Orange, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA).
KLRG1 is a glycosylated homodimer (30–38 kDa) containing an ITIM-like cytoplasmic motif. It is expressed on approximately one-third of mouse NK cells and a subset of T cells. Expression is regulated by MHC class I molecules via Ly49 and SHP-1 signaling.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimize titration for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is compatible with cytometers equipped with a 355 nm UV laser. Protect from light to prevent photo-induced oxidation.

Staining Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when using multiple polymer dye-conjugated antibodies to minimize non-specific interactions.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric lectin-like type 2 transmembrane receptor containing ITIM motifs that interact with SHP-1 phosphatase.
It functions as an inhibitory receptor expressed on NK cells and certain T cells, which are involved in tumor and virus-infected cell lysis and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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