Thermo Fisher Scientific eIF4A3 Recombinant Rabbit Monoclonal Antibody (17H5L19)
Recombinant rabbit monoclonal antibody recognizing eIF4A3 in human and mouse. Validated for WB and ICC/IF. Offers high specificity, reproducibility, and consistent performance. Produced using in vitro expression for animal origin-free formulation.
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- 702545
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- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 17H5L19 |
| Immunogen | Peptides corresponding to human eIF4A3 (aa 15–33, 302–318) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2664256 |
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Sheep, Pig, and Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems utilize cloned antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
eIF4A3 is a member of the DEAD box protein family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). These proteins are putative RNA helicases involved in processes such as translation initiation, RNA splicing, and ribosome/spliceosome assembly. eIF4A3 is a nuclear matrix protein with high sequence similarity to eIF4AI and eIF4AII, other members of the DEAD box family. It plays roles in embryogenesis, spermatogenesis, and cellular growth/division.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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