Thermo Fisher Scientific Phospho-MECP2 (Ser421) Polyclonal Antibody
Phospho-MECP2 (Ser421) 폴리클로날 항체로, Western blot, IHC, ICC/IF에 사용 가능. Mouse와 Rat에 반응하며, Rabbit IgG 유래. 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. 인간 및 영장류에도 반응 예측됨.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.25 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.1 mg/mL BSA, 50% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2552706 |
Product Specific Information
- This antibody is predicted to react with human and non-human primate based on 100% sequence homology.
- Contains enough material to conduct approximately 10 mini-Western blots.
Target Information
MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that contain a methyl-CpG binding domain. MECP2 binds specifically to methylated DNA, a key modification in eukaryotic genomes essential for mammalian development. It can repress transcription from methylated gene promoters. Unlike other MBD family members, MECP2 is X-linked and subject to X inactivation. It is dispensable in stem cells but essential for embryonic development. Mutations in the MECP2 gene are the primary cause of Rett syndrome, a progressive neurological developmental disorder and a common cause of mental retardation in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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