Thermo Fisher Scientific Phospho-MYPT1/MYPT2 (Ser668, Ser618) Polyclonal Antibody
MYPT1/MYPT2 단백질의 Ser668/Ser618 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 적용 가능. 고순도, 1 mg/mL 농도, PBS/glycerol 버퍼에 보관. 연구용 전용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PPP1R12A (Accession O14974), corresponding to amino acid residues around phosphorylated Ser668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2903264 |
Product Specific Information
Antibody detects endogenous levels of MYPT1/2 only when phosphorylated at Ser668/Ser618.
Target Information
Myosin phosphatase target subunit 1 (MYPT1), also known as the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase.
Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho.
The active form of RhoA (GTP.RhoA) interacts with the myosin-binding subunit (MBS) of myosin phosphatase, controlling the extent of phosphorylation of myosin light chain (MLC).
Rho-associated kinase (Rho-kinase), activated by GTP.RhoA, phosphorylates MBS and consequently inactivates myosin phosphatase.
Overexpression or activation of RhoA increases phosphorylation of MBS and MLC, indicating that Rho inhibits myosin phosphatase through Rho-kinase.
Several transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-118764_MYPT1MYPT2_O60237-1_Rabbit.svg)
(이미지: PA5-118764_MYPT1MYPT2_O60237-1_Rabbit_PDP.jpeg)
(이미지: PA5-118764_MYPT1MYPT2_O14974-1_Rabbit.svg)
(이미지: PA5-118764_MYPT1MYPT2_O14974-1_Rabbit_PDP.jpeg)
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