Thermo Fisher Scientific Mouse anti-Rat IgG2a Secondary Antibody, eFluor 615, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• eFluor 570
• eFluor 660
• FITC
• PE
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a secondary reagent in flow cytometry and microscopy.
It does not cross-react with other rat isotypes or mouse antibodies.

Applications Reported:
Immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections, and immunocytochemistry.

Applications Tested:
Validated for immunocytochemistry and immunohistochemistry using a rat primary antibody at 10 µg/mL.
Not validated for flow cytometry.

Filter Recommendation:
Use a filter capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75).
A standard Alexa Fluor™ 594 filter is acceptable.

Excitation / Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Rat secondary antibodies are affinity-purified with high specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies enhance versatility and sensitivity through multiple binding events and signal amplification.
Typically produced by immunizing host animals with pooled rat immunoglobulins, followed by purification and conjugation for high specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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