Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Brilliant Violet 786, eBioscience
마우스 CD69 단백질을 인식하는 H1.2F3 단클론 항체로, Brilliant Violet 786 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 활성화된 T 및 B 세포 표면의 CD69 발현 분석에 적합합니다. 광민감성이 있으므로 어둡게 보관해야 합니다.
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Applications
- Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Violet™ 786, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1.2F3 |
| Conjugate | Brilliant Violet™ 786 |
| Excitation / Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937221 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer on lymphocyte surfaces. Activation of T and B cells rapidly upregulates CD69, implicating it in lymphocyte activation and development.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes; ≤0.5 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize titer for best performance.
Brilliant Violet™ 786 (BV786) emits at 786 nm and is excited by a violet (405 nm) laser. Ensure instrument compatibility.
When using multiple Brilliant Violet™, Brilliant Ultra Violet™, or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Light sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
Not compatible with methanol-based fixation.
- Excitation: 407 nm
- Emission: 786 nm
- Laser: Violet Laser
Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. CD69 expression is induced upon T lymphocyte activation and may play roles in proliferation and signal transduction in NK cells and platelets.
Associated diseases include coccidiodomycosis and asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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