Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer on lymphocyte surfaces. Activation of T and B cells rapidly upregulates CD69, implicating it in lymphocyte activation and development.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; ≤0.5 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize titer for best performance.

Brilliant Violet™ 786 (BV786) emits at 786 nm and is excited by a violet (405 nm) laser. Ensure instrument compatibility.

When using multiple Brilliant Violet™, Brilliant Ultra Violet™, or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
Not compatible with methanol-based fixation.

• Excitation: 407 nm
• Emission: 786 nm
• Laser: Violet Laser

Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. CD69 expression is induced upon T lymphocyte activation and may play roles in proliferation and signal transduction in NK cells and platelets.
Associated diseases include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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