Thermo Fisher Scientific MAP2K3 Monoclonal Antibody (OTI2A7), TrueMAB
MAP2K3 단백질을 인식하는 Mouse 모노클로날 항체로, WB, IHC, ICC/IF에 사용 가능. 고순도 친화 크로마토그래피 정제, 무보존제 PBS 버퍼에 동결건조 형태로 제공. 연구용으로 세포 신호전달 및 MEK3 관련 연구에 적합.
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Thermo Fisher Scientific MAP2K3 Monoclonal Antibody (OTI2A7), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A7 |
| Immunogen | Full-length human recombinant MAP2K3 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting method: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction pathways and is required for the expression of glucose transporter. It phosphorylates and activates MAPK14/p38-MAPK.
Activation can occur via insulin and is linked to oncogenic transformation through RAS expression.
Inhibition of MAP2K3 is implicated in the pathogenesis of Yersinia pseudotuberculosis.
Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported for MEK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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