Thermo Fisher Scientific beta Catenin Monoclonal Antibody (9F2)
Human 및 Mouse 시료에서 beta-catenin 단백질을 검출하는 마우스 단클론 항체. Western blot, ICC/IF, Immunoprecipitation에 적합. Protein G 정제, PBS/BSA buffer에 보관. 세포 부착 및 신호전달 연구에 활용 가능.
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Thermo Fisher Scientific beta Catenin Monoclonal Antibody (9F2)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 1 publication |
| Immunoprecipitation (IP) | 1 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F2 |
| Immunogen | 120 kDa MBP/beta catenin fusion protein purified on amylose column |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_326078 |
Product Specific Information
MA1-2001 detects beta-catenin from human samples.
It has been successfully used in Western blot procedures, detecting an ~94 kDa protein representing beta-catenin from A431 cell extract.
MA1-2001 is also suitable for immunoprecipitation and immunofluorescence assays.
The immunogen is a 120 kDa MBP/beta-catenin fusion protein purified via an amylose column.
Target Information
Beta-catenin is an adherens junction (AJ) protein involved in cell-cell adhesion and epithelial layer maintenance. It interacts with E-cadherin and alpha-catenin to link cell adhesion complexes to the actin cytoskeleton.
Beta-catenin also associates with signaling complexes containing adenomatous polyposis coli protein and microtubules, participating in pathways involving tyrosine kinases, phosphatases, and Wnt/Wingless signaling.
It plays roles in morphogenesis and is expressed in various hair follicle and epithelial cells.
Pathologically, beta-catenin is implicated in pilomatrixoma, medulloblastoma, colorectal cancer, ovarian cancer, and other solid tumors.
In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors, promoting expression of genes such as cyclin D and c-myc, which drive cell proliferation and tumorigenesis.
Usage Note
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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