Thermo Fisher Scientific alpha Catenin Monoclonal Antibody (1G5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1G5

Immunogen

90 kDa MBP/alpha catenin fusion protein purified on amylose column.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_326077

Product Specific Information

MA1-2000 detects alpha catenin from human samples.

MA1-2000 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~102 kDa protein representing alpha catenin from scc-1 cell extract. In Western blot, this antibody did not cross-react with mouse samples. MA1-2000 can also be used in immunoprecipitation and immunofluorescence procedures.

The MA1-2000 immunogen is a 90 kDa MBP/alpha catenin fusion protein purified via an amylose column.

Target Information

Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.