Thermo Fisher Scientific alpha Catenin Monoclonal Antibody (1G5)
인간 알파 카테닌을 검출하는 Mouse IgG1 단일클론 항체. Western blot, ICC/IF, IP에 사용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, Protein G로 정제됨. -20°C 보관, 반복 동결/해동 피해야 함.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | View 5 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1G5 |
| Immunogen | 90 kDa MBP/alpha catenin fusion protein purified on amylose column |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_326077 |
Product Specific Information
MA1-2000 detects alpha catenin from human samples.
It has been successfully used in Western blot procedures, detecting an ~102 kDa protein representing alpha catenin from SCC-1 cell extract.
This antibody does not cross-react with mouse samples in Western blot.
MA1-2000 can also be used in immunoprecipitation and immunofluorescence procedures.
The immunogen is a 90 kDa MBP/alpha catenin fusion protein purified via an amylose column.
Target Information
Alpha catenin is a member of the β-catenin family of cytosolic proteins.
It interacts with N-cadherin and β-catenin on the cytosolic side of adherens junctions to mediate the interaction with the actin cytoskeleton.
Alpha catenin forms a homodimer in solution but dissociates to form a 1:1 heterodimer with β-catenin to interact with cadherins.
It regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development, and functions in the control of startle modulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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