Thermo Fisher Scientific E2F4 Polyclonal Antibody
Thermo Fisher Scientific의 E2F4 Polyclonal Antibody는 인간 E2F4 단백질(363-413 aa)을 면역원으로 한 Rabbit Polyclonal 항체입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 363 and 413 of human E2F transcription factor 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides. Differential phosphorylation contributes to the microheterogeneity of E2F-4 (total aa 411–416, varies due to a trinucleotide repeat).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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