Thermo Fisher Scientific Phospho-PKC delta (Ser645) Polyclonal Antibody
PKC delta 인산화형(Ser645)을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P)에서 사용 가능. 항원 친화 정제 방식으로 제조되었으며, 1mg/mL 농도의 액상 형태. U937 세포에서 약 77kDa 밴드를 검출하며, 연구용으로 적합.
- 카탈로그번호
- PA128462
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from the region of human PKC delta containing serine 645 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_1959685 |
Product Specific Information
- Recommended positive control: U937 cells
- Detects a band approximately 77 kDa
Target Information
The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways.
PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and distinct cellular roles. The protein encoded by this gene is one of the PKC family members. Studies in humans and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of various cell types. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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