Thermo Fisher Scientific Phospho-PKC delta (Ser645) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:100

Product Specifications

Product Specific Information

• Recommended positive control: U937 cells
• Detects a band approximately 77 kDa

Target Information

The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, response to extracellular signals, and substrate specificity.

PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways.

PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and distinct cellular roles. The protein encoded by this gene is one of the PKC family members. Studies in humans and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of various cell types. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.