Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE-Cyanine7, eBioscience
인간 및 생쥐 ZAP70/SYK 단백질의 인산화형(Tyr319/Tyr352)을 검출하는 단클론 항체. PE-Cyanine7 형광 표지로 유세포분석에 적합. Affinity chromatography로 정제되었으며, 4°C 암소 보관. TCR/BCR 신호전달 연구에 활용 가능.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.06 µg)/test
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | n3kobu5 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811791 |
Available Formats:
Product Specific Information
This n3kobu5 monoclonal antibody recognizes human and mouse ZAP-70 and SYK when phosphorylated on Y319 and Y352, respectively.
ZAP-70 and SYK are members of the SYK protein tyrosine kinase family that are phosphorylated and activated by Src family PTKs.
Phosphorylation of ZAP-70 Y319 by Lck is essential for TCR-dependent signaling, while SYK Y352 phosphorylation by Fyn/Lyn is critical for BCR signaling and B-cell development.
- Specificity Determination: ELISA, Flow Cytometry, Western Blotting
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Pre-diluted and validated for intracellular staining of sodium pervanadate-treated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00)
- Recommended Protocol: See Staining Intracellular Antigens for Flow Cytometry
- Recommended Use: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Light Sensitivity: Protect from light; tandem dye is sensitive to photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product # 00-8222-49)
- 1-step Fix/Lyse Solution (Product # 00-5333-57)
Fluorescence Characteristics:
- Excitation: 488–561 nm
- Emission: 775 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase in the Syk family, expressed in T-cells and NK cells, and essential for T-cell receptor activation.
Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319.
- Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhance lymphocyte signaling
- Tyr292 attenuates transient activation of ZAP-70
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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