Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 1 publication

Product Specifications

Available Formats:

• APC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This n3kobu5 monoclonal antibody recognizes human and mouse ZAP-70 and SYK when phosphorylated on Y319 and Y352, respectively.
ZAP-70 and SYK are members of the SYK protein tyrosine kinase family that are phosphorylated and activated by Src family PTKs.
Phosphorylation of ZAP-70 Y319 by Lck is essential for TCR-dependent signaling, while SYK Y352 phosphorylation by Fyn/Lyn is critical for BCR signaling and B-cell development.

• Specificity Determination: ELISA, Flow Cytometry, Western Blotting
• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Pre-diluted and validated for intracellular staining of sodium pervanadate-treated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00)
• Recommended Protocol: See Staining Intracellular Antigens for Flow Cytometry
• Recommended Use: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Light Sensitivity: Protect from light; tandem dye is sensitive to photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-57)

Fluorescence Characteristics:

• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase in the Syk family, expressed in T-cells and NK cells, and essential for T-cell receptor activation.
Upon TCR engagement, ZAP-70 is phosphorylated on tyrosines 292, 315, and 319.

• Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhance lymphocyte signaling
• Tyr292 attenuates transient activation of ZAP-70

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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