
Thermo Fisher Scientific MGMT Monoclonal Antibody (OTI6C4), TrueMAB
MGMT 단백질에 특이적인 Mouse IgG1 단클론 항체로, Western Blot 및 IHC(P) 실험에 적합. 인간 시료 반응성 확인됨. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. DNA 손상 복구 관련 연구용으로 최적화된 항체.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6C4 |
| Immunogen | Full length human recombinant MGMT protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve ~1 mg/mL antibody concentration.
- For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is a DNA repair protein that confers resistance to alkylating chemotherapeutic agents by removing alkyl groups from the O6 position of guanine. It protects normal cells from genotoxic and carcinogenic effects of DNA alkylation. MGMT expression levels vary by cell type, tissue, species, and tumor characteristics.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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