
Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
TRITC 형광 표지를 가진 염소 유래 F(ab')2 항체로 Rat IgG(H+L)에 특이적입니다. Western blot, IHC, ELISA에 사용 가능하며 높은 교차 흡착으로 비특이적 반응 최소화. Lyophilized 형태로 안정적이며, 552/578 nm에서 발광합니다.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation / Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536026 |
| Target | IgG |
| Cross Adsorption | Against bovine, goat, human, mouse and rabbit IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life is 1 year from date of receipt. Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins, IgG from goat, hamster, human, mouse, or rabbit, or serum from bovine, goat, horse, human, mouse, or rabbit.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in detection, sorting, or purification of their target. Secondary antibodies offer versatility for multiple detection systems (e.g., HRP, AP, fluorescence) and provide greater sensitivity through signal amplification. They are produced by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (immunoaffinity chromatography, fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- HRP
- Request custom conjugation
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