Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
TRITC로 결합된 염소 유래 항-쥐 IgG(F(ab')2) 2차 항체로, 높은 교차 흡착 처리로 비특이적 반응 최소화. 면역세포염색(ICC/IF)에 적합하며 552/578 nm에서 여기/방출. 동결건조 형태로 제공되며 4°C 또는 -20°C에서 보관.
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Applications
- Immunocytochemistry (ICC/IF): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536028 |
| Target | IgG |
| Cross Adsorption | Against bovine, goat, human, mouse, and rabbit IgG |
| Antibody Form | F(ab')2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins, or IgG/serum from goat, hamster, human, mouse, rabbit, bovine, or horse.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity through multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, or conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- HRP
- Custom conjugation available upon request
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