Thermo Fisher Scientific CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), FITC, eBioscience
마우스 및 랫트 CD61(Integrin β3)을 인식하는 FITC 결합 단클론 항체입니다. 유세포분석(Flow Cytometry)에 적합하며, 488 nm 레이저에서 520 nm 방출을 보입니다. 세포 부착 및 신호전달 연구에 유용하며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 1 µg/test | 11 |
| Neutralization (Neu) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C9.G3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465105 |
Additional Available Formats
- Unconjugated
- APC
- Biotin
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 725
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- NovaFluor Yellow 730
- NovaFluor Yellow 755
- PE
- Custom conjugation available
Product Specific Information
Description:
The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61 (integrin beta3). CD61 is expressed by activated T cells, granulocytes, and platelets. It associates non-covalently with integrin alpha subunits alphaV (CD51) to form the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes mediate adhesion to extracellular matrix components such as fibrinogen, fibronectin, vitronectin, thrombospondin, and von Willebrand factor.
Applications Reported:
The 2C9.G3 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. Recommended ≤1 µg/test. One test is defined as the amount of antibody required to stain a cell sample in 100 µL final volume. Cell number may vary (10⁵–10⁸ cells/test). Optimal titration is recommended for each assay.
Spectral Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD61 (GPIIIa, ITGB3) is a glycoprotein expressed on megakaryocytes, platelets, and their precursors. It functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, and vitronectin, and plays a key role in platelet aggregation and adhesion. CD61 forms heterodimers with integrin alphaIIb (CD41) or alphaV (CD51). It participates in cell adhesion and signal transduction. Disorders associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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