Thermo Fisher Scientific QKI Monoclonal Antibody (N147/6)
QKI 단백질을 검출하기 위한 Mouse monoclonal antibody (Clone N147/6). Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 시료에 반응. Protein G로 정제된 1 mg/mL 액상 시약으로, -20°C에서 보관. 다양한 형광 표지 형식으로도 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N147/6 |
| Immunogen | Fusion protein amino acids 1–341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical). >90% identity with QKI-6, QKI-7, and QKI-7b. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2735341 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-27651 was sufficient for detection of Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 36–38 kDa.
This antibody was formerly sold as clone S147-6.
Target Information
This gene encodes the sacsin protein, which includes a UbL domain at the N-terminus, a DnaJ domain, and a HEPN domain at the C-terminus. The gene is highly expressed in the central nervous system, also found in skin, skeletal muscles, and at low levels in the pancreas. This gene includes a very large exon spanning more than 12.8 kb. Mutations in this gene result in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset cerebellar ataxia with spasticity and peripheral neuropathy. Sacsin has been found to protect against mutant ataxin-1. A pseudogene associated with this gene is located on chromosome 11. Alternative splicing of this gene results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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