Thermo Fisher Scientific FICD Monoclonal Antibody (OTI3G3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882) to ensure carrier-free conditions.

Target Information

FICD is a dual-function enzyme capable of mediating both AMPylation and de-AMPylation of target proteins, depending on the cellular context. The Glu-231 residue determines whether it acts as an AMPylase or de-AMPylase. It regulates the ERN1/IRE1-mediated unfolded protein response (UPR) by modifying HSPA5/BiP.

• In unstressed cells: acts as an adenylyltransferase, AMPylating HSPA5/BiP at Thr-518, thereby inactivating it.
• Under ER stress: acts as a phosphodiesterase, removing AMP from HSPA5/BiP at Thr-518 to restore activity.
  Although FICD can AMPylate RhoA, Rac, and Cdc42 in vitro, these are not physiological substrates.
  (Source: UniProt)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.