Takara Dr. GenTLE (gene trapping by liquid extraction) systems

Takara Bio`s Dr. GenTLE (Gene Trapping by Liquid Extraction) systems are designed for high quality and downstream application-ready DNA extraction and purification. Two Dr. GenTLE systems are available: 1) Dr. GenTLE (from Yeast) High Recovery (Cat. # 9082) for efficient extraction of genomic DNA from yeast cells and 2) Dr. GenTLE Precipitation Carrier (Cat. # 9094) for high efficiency ethanol precipitation of nucleic acid solutions.

Takara Bio`s Dr. GenTLE (Gene Trapping by Liquid Extraction) systems are designed for high quality and downstream application-ready DNA extraction and purification. Two Dr. GenTLE systems are available: 1) Dr. GenTLE (from Yeast) High Recovery (Cat. # 9082) for efficient extraction of genomic DNA from yeast cells and 2) Dr. GenTLE Precipitation Carrier (Cat. # 9094) for high efficiency ethanol precipitation of nucleic acid solutions.

Dr. GenTLE (from Yeast) High Recovery includes reagents required for DNA extraction and purification from a variety of yeasts (see below) via cell wall digestion and cell lysis followed by salting-out for DNA purification. Genomic DNA isolated with this kit can be used for many applications including DNA sequencing, Southern blot analysis, cloning, PCR, and restriction enzyme digestion. Most standard procedures using 2 x 108 yeast cells obtain 4–10 µg of genomic DNA (35–200 kb).

Dr. GenTLE Precipitation Carrier allows for higher DNA/RNA recovery versus glycogen-based precipitation, even from dilute (5 ng/ml) nucleic acid samples, taking advantage of a high-efficiency ethanol precipitation step during nucleic acid purification.

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Overview

Applications

• Dr. GenTLE (from Yeast) High Recovery

  • Quick and consistent yeast genomic DNA extraction in just 40 minutes
  • Downstream application-ready nucleic acids

• Dr. GenTLE Precipitation Carrier

  • Precipitation of DNA/RNA from even dilute solutions (5 ng/ml nucleic acid)
  • Consistent, high-throughput nucleic acid preparation
  • DNA/RNA recovery from rare and unique samples

Components

• Dr. GenTLE (from Yeast) High Recovery

  Dr. GenTLE Yeast Solution A	15 ml
  Dr. GenTLE Yeast Solution B	3 ml
  Dr. GenTLE Yeast Solution C	6 ml
  TE Buffer	6 ml

• Dr. GenTLE Precipitation Carrier

  Dr. GenTLE Precipitation Carrier	1 x 800 µl
  3M CH3COONa	2 x 1 ml

More Information

Dr. GenTLE (from Yeast) High Recovery Compatible Yeast

• Yeast from which DNA can be prepared

  Ashbya	Hanseniaspora	Metschikowia	Schizosaccharomyces
  Candida	Hansenula	Pullularia	Selenozyma
  Debaryomyces	Kloeckera	Saccharomyces	Trigonopsis
  Endomyces	Kluyveromyces	Saccharomycopsis	Wickerhamia
  Eremothecium	Lipomyces	Saccharomycodes

• Yeast from which DNA can be prepared (Strain-dependent)

  Bretanomyces	Nadsonia	Rodosporidium	Stephnoascus
  Cryptococcus	Pichia	Schwanniomyces	Torulopsis

• Yeast from which DNA cannot be prepared

  Bullera	Rhosotorula	Sporobolomyces	Trichosporon
  Pityrosporum	Sporidiobolus	Stetigmatomyces

Storage

• Dr. GenTLE (from Yeast) High Recovery: Store all components other than Dr. GenTLE Yeast Solution B at 4°C. Store Dr. GenTLE Yeast Solution B at room temperature as low temperature storage may result in formation of a precipitant. If a precipitate forms in Dr. GenTLE Yeast Solution B, dissolve by heating the solution at 37°C just before use.
• Dr. GenTLE Precipitation Carrier: Room temperature.