Thermo Fisher Scientific HMHA1 Polyclonal Antibody
Human HMHA1 단백질을 인식하는 Goat Polyclonal Antibody로 Western blot 및 IHC(Paraffin)에서 검증됨. 0.5 mg/mL 농도의 액상 형태로 제공되며, TBS(pH 7.3) 버퍼와 0.5% BSA, 0.02% sodium azide를 포함. RhoGAP 관련 단백질 연구 및 minor histocompatibility antigen 연구에 적합.
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- PA5142852
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.05 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence KATGTLKRPTSLSRH-C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2933495 |
Product Specific Information
- This antibody is designed to recognize the whole protein as coded by NP_036424.2 and will not recognize the peptide found eluted from HLA-A 0201 molecules by den Haan et al. (Science, 1998 Feb 13;279(5353):1054-7, PMID: 9461441).
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
HMHA1 contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain, which can negatively regulate the actin cytoskeleton and cell spreading. The N-terminal BAR-domain can exert an autoinhibitory effect on RhoGAP activity. It is a precursor of the histocompatibility antigen HA-1, one of the minor histocompatibility antigens (mHags) that can elicit immune responses when complexed with MHC molecules.
Minor histocompatibility antigens are peptides derived from polymorphic intracellular proteins. When bound to MHC class I or II molecules and expressed on the cell surface, they can stimulate T-cell responses, potentially triggering graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical donors.
Mismatch of mHag HA-1 is associated with severe GVHD following HLA-identical bone marrow transplantation (BMT). HA-1 is presented by MHC class I HLA-A*0201 and other HLA-A alleles, eliciting cytotoxic T-lymphocyte (CTL) reactivity against hematologic malignancies post-transplant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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