Thermo Fisher Scientific PGCF Polyclonal Antibody
Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능한 Rabbit Polyclonal Antibody. Human, Mouse, Rat에 반응하며, 합성 펩타이드 면역원으로 제작됨. 친화 크로마토그래피로 정제된 액상 항체로, -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic C-terminal peptide corresponding to unique amino acid sequences on Particulate Guanylyl Cyclase F protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
GUCY2F (Guanylate cyclase 2F, retinal) — also known as GUC2F or RETGC2 — belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
GUCY2F may play a specific functional role in the rods and/or cones of photoreceptors and may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction.
By in situ hybridization, mRNA encoding GUCY2F is found only in the outer nuclear layer and inner segments of photoreceptor cells.
Using synthetic peptide antiserum specific for each RetGC subtype, RetGC-2 (GUCY2F) can be distinguished from RetGC-1 as a slightly smaller protein in immunoblots of bovine rod outer segments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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