Thermo Fisher Scientific CIDEC Polyclonal Antibody
Thermo Fisher Scientific의 CIDEC Polyclonal Antibody는 인간 및 생쥐 시료에서 CIDE-3 단백질을 검출하는 토끼 유래 IgG 항체입니다. Western blot에 적합하며, 93% 서열 상동성을 가진 FSP27에도 교차 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, 2–8°C에서 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues L(10)LYPKSLSRHVSVRTS(25) of human CIDE-3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | 2–8°C for up to 3 months; for long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2079998 |
Product Specific Information
- PA1-4316 detects the human CIDE-3 protein in human samples.
- Expected to recognize mouse samples due to 93% sequence homology.
- Successfully used in Western blot procedures.
- Detects a ~27 kDa protein representing human CIDE-3 in HepG2 cell lysate.
- Cross-reactivity observed with FSP27 (mouse homolog of CIDE-3, 93% sequence identity).
- Recommended storage: 4°C for 3 months or -20°C for longer periods. Avoid repeated freeze-thaw cycles.
Target Information
DNA fragmentation is a critical step in apoptosis, induced by DNA fragmentation factor (DFF), composed of two subunits: a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45).
A novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified, including human CIDE-3, a novel member of this family.
Two transcripts, CIDE-3 and CIDE-3α, are present in HepG2 and A375 cells.
CIDE-3 is localized at chromosome 3p25, a region associated with high-frequency loss of heterozygosity in many tumors, suggesting a potential role in tumorigenesis prevention.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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