Thermo Fisher Scientific Cullin 5 Polyclonal Antibody
Western blot 및 IHC(P)에서 사용 가능한 Rabbit Polyclonal 항체로, Human, Mouse, Rat 반응성을 가집니다. Affinity chromatography로 정제되었으며 PBS/glycerol buffer에 보관됩니다. CUL5 단백질의 내인성 발현을 검출합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CUL5 (Accession Q93034), corresponding to amino acid residues A375–L425 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2852141 |
Product Specific Information
Antibody detects endogenous levels of total CUL5.
Target Information
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.
As a scaffold protein, it may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component.
ECS(SOCS1) seems to direct ubiquitination of JAK2. It may also be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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