Thermo Fisher Scientific Placental Alkaline Phosphatase Monoclonal Antibody (8B6), eFluor 660, eBioscience
인간 PLAP(Placental Alkaline Phosphatase)을 인식하는 단일클론 항체로, IHC, ICC, ELISA 등에 사용 가능. eFluor 660 형광 표지로 적색 레이저(633 nm)에서 검출. 인간 배아줄기세포 및 생식세포 종양 연구에 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 20 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 20 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8B6 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574402 |
Additional Formats
Product Specific Information
The monoclonal antibody 8B6 recognizes human Placental Alkaline Phosphatase (PLAP), a 130 kDa enzyme expressed by placental syncytiotrophoblastoid cells. This enzyme exhibits slightly different kinetics from other phosphatases and can be found both on the plasma membrane and in the cytoplasm. Expression occurs in several germ line cancers (ovarian, testicular), human embryonic stem cells, and bone marrow-derived mesenchymal stromal cells.
This antibody reacts with both Regan and Nagao isozymes.
Applications Reported: Suitable for IHC (frozen and paraffin-embedded tissues) and ELISA.
Applications Tested: Tested on FFPE human placenta with trypsin digestion. Recommended ≤20 µg/mL; titration advised for optimal performance.
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647. It emits at 659 nm and is excited with a red laser (633 nm). Ensure instrument compatibility.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Placental Alkaline Phosphatase (PLAP) regulates specific inflammatory processes and exists as one of four related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney forms. PLAP (Regan and Nagao type) is a membrane-bound isoenzyme expressed in the placenta during the third trimester. It aids in identifying testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors express epithelial membrane antigen (EMA).
PLAP may play a role in matrix mineralization, though mice lacking this enzyme show normal skeletal development. Mutations in PLAP are linked to hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects, with variable severity based on mutation type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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