Thermo Fisher Scientific Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eFluor 660, eBioscience

Thermo Fisher Scientific Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA), a cell surface glycoprotein predominantly expressed on T cells in the skin. CLA can also be detected on peripheral T and NK cells, monocytes, neutrophils, and CD1a+ dendritic cells. The antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex), which are involved in adhesion and infiltration of T lymphocytes at sites of cutaneous inflammation.

HECA-452 has been reported to inhibit sLex binding to E-selectin, though this remains debated.
Applications include flow cytometry, western blotting, and immunohistochemical staining of FFPE tissue sections. Tested by flow cytometry on normal human peripheral blood cells and by IHC on FFPE human tissue at ≤20 µg/mL. Optimal performance with low pH antigen retrieval; high pH retrieval also applicable.

eFluor™ 660 replaces Alexa Fluor™ 647, emitting at 659 nm and excited by the red laser (633 nm).
Ensure instrument compatibility for detection.
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtered post-manufacturing through 0.2 µm membrane.

Target Information

Cutaneous Lymphocyte Antigen (CLA) is mainly expressed on skin T cells but can also be found on peripheral T and NK cells, monocytes, neutrophils, and CD1a+ dendritic cells. It recognizes sialofucosylated glycans and sialyl Lewis x (sLex), binding to E-selectin and ELAM-1, mediating lymphocyte adhesion and infiltration in skin inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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