Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), eFluor 660, eBioscience
인간 및 마우스 IRF4 단백질을 인식하는 단클론 항체로, eFluor 660 형광 표지. Flow cytometry, IHC, ELISA, ChIP assay에 사용 가능. B세포, T세포, 대식세포 기능 연구에 적합. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | 16 publications |
| ELISA | - | 1 publication |
| ChIP assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E4 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574393 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- Brilliant Violet 480
- Brilliant Violet 650
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.
In B cells, IRF4 is highly expressed in mature plasma cells and regulates differentiation through interaction with PU.1 and activation of genes such as Prdm1 (Blimp1).
In T cells, IRF4 contributes to Treg, Th2, Th9, and Th17 cell development and function, being upregulated upon activation. It induces Blimp1 and IL-10 expression in Tregs.
IRF4 also plays a role in macrophage polarization and immune regulation, and its overexpression has been linked to several hematologic malignancies.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Intracellular staining and flow cytometry of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set.
Target Information
The interferon regulatory factor (IRF) family contains transcription factors with a conserved tryptophan pentad repeat DNA-binding domain.
IRFs regulate interferon expression in response to viral infection and control interferon-inducible gene expression.
IRF4 is lymphocyte-specific and negatively regulates toll-like receptor (TLR) signaling, a key pathway in innate and adaptive immunity.
A chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH loci is associated with multiple myeloma.
Alternative splicing variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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