Thermo Fisher Scientific Carbonic Anhydrase IX Monoclonal Antibody (SP106)
Human CA IX 단백질을 인식하는 Rabbit Monoclonal Antibody (Clone SP106). Western blot, IHC(P), Flow Cytometry에 적합. Protein A/G로 정제된 액상 포맷으로 제공. Hypoxia 관련 연구 및 CA IX 발현 분석에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP106 |
| Immunogen | Synthetic peptide derived from human CA-9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.11 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537932 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes. Following antigen retrieval, incubate samples with the primary antibody for 10 minutes at room temperature. A suggested positive control is stomach tissue.
Target Information
Carbonic anhydrase (CA) is an enzyme that assists in the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is one of the most inducible genes under hypoxic conditions due to its stability and membrane localization. Carbonic anhydrases play a crucial role in regulating pH in normal tissues by controlling hydrogen ion (H⁺) flux.
Since pH regulation is important for cell survival under hypoxia, inhibition of CA IX leads to increased cell death in hypoxic environments. Therefore, CA IX serves as a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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