Thermo Fisher Scientific ATRX Polyclonal Antibody
ATRX 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), PLA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 버퍼에 보존. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| in situ PLA (PLA) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 2161–2443 of Rad54 (Uniprot ID# P46100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.27 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152865 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH3T3, PC-12, Rat-2
- Predicted reactivity: Mouse (97%), Chimpanzee (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATRX encodes a protein containing an ATPase/helicase domain, belonging to the SWI/SNF family of chromatin remodeling proteins. Mutations in this gene are associated with X-linked mental retardation (XLMR) often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations can alter DNA methylation patterns, linking chromatin remodeling, DNA methylation, and gene expression in development. The protein undergoes cell cycle-dependent phosphorylation, regulating its nuclear matrix and chromatin association, suggesting roles in gene regulation and chromosomal segregation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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