Thermo Fisher Scientific CD79a Recombinant Rabbit Monoclonal Antibody (IGA, 1688R)
CD79a 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며 HEK293 세포에서 발현되었습니다. 단백질 A로 정제된 액상 항체로, 4°C 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | IGA, 1688R |
| Immunogen | Synthetic peptide corresponding to aa 202–216 (GTYQDVGSLNIADVQ) of human CD79a protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD79a is a disulfide-linked heterodimer that includes the B29 (CD79b) polypeptide. It is a B lymphocyte antigen receptor component associated with Ig-alpha and Ig-beta, essential for B-cell antigen receptor expression and function. CD79a appears at the pre-B cell stage and persists until the plasma cell stage, where it remains as an intracellular component. It is expressed in most acute leukemias of precursor B-cell type, B-cell lines, B-cell lymphomas, and some myelomas, but not in myeloid or T-cell lines.
Diseases associated with CD79a include Agammaglobulinemia 3 and Agammaglobulinemia, Non-Bruton type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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